Data/hora: 28/05/2024 15:10:19

Biblioteca(s): | Embrapa Caprinos e Ovinos; Embrapa Pantanal.
Data corrente: | 25/04/1994
Data da última atualização: | 18/07/2018
Autoria: | ALVES, S. F.
Afiliação: | University of California (Davis).
Título: | Immunochemical and protection studies analyse of Leptospira.
Ano de publicação: | 1993
Fonte/Imprenta: | Davis: University of California, 1993.
Páginas: | 86p.
Idioma: | Inglês
Notas: | Ph.D. Dissertation.
Conteúdo: | Leptospira interrogans is the causative agent of leptospirosis, an important widespread zoonosis. The control of this disease is very difficult due to a variety of factors, including the lack of a vaccine which provides protection against renal infection and leptospiruria. New approaches for vaccine formulation or improvement of the current vaccine are needed. Important and immunological relevant targets should be identified which provide a protective immune response. In the first chapter we used Triton X-114 to isolate and identify common outer envelope proteins from prevalent Leptospira serovars. These proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting and demonstrated to be expressed and immunologically conserved in all serovars tested. Surface proteolysis, immunoprecipitation and immunofluorescence were utilized to determine the location of these proteins in the cell. Our findings suggest that these antigens are present on the outer surface of Leptospira. Internal sequencing of fragments were obtained after enzymatic or chemical digestion of these proteins. The relatedness of each protein among the serovars was assessed by protein fingerprinting. These proteins are expressed and highly conserved among prevalent Leptospira serovars. In chapter II, polyclonal antibodies to whole cell, detergent phase proteins an gel purified proteins were used in a bactericidal assay and in passive protection studies with immunosuppressed Balb/c mice. Bactericidal activity was observed in all serovars when antiserum to whole cell or detergent phase proteins were used. In addition, antiserum to gel purified proteins from avirulent Leptospira of serovar pomona demonstrated bactericidal activity on virulent organisms of this same serovar. Total protection was obtained in mice when antibodies against whole cell and detergent phase proteins were used. Protection was defined as prevention of kidney infection in challenged mice. Partial protection was obtained with antibodies raised against 2 of the detergent phase proteins (32 and 40 kDa).
Palavras-Chave: | Disease; Immunochemical; Imunoquimica; Pathology; Patologia; Protein; Vaccine; Virulencia.
Thesagro: | Doença; Imunização; Leptospirose; Proteína; Vacina; Zoonose.
Thesaurus Nal: | immunity; Leptospira; Leptospira interrogans; Leptospirosis.

Categoria do assunto:

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H Saúde e Patologia

Registro original: | Embrapa Pantanal (CPAP)

Exemplares:

CNPC - (2254-1) - ADD - TS - PP - TS 1993.00005 - ALV - 1993.00005 - EMP
CPAP - (32095-1) - ADD - TS - PP - TS94.00004 -  - 1994.00004 - DIS